亚洲欧美国产vr在线观,暗交小拗女一区二区三区,国产熟女出轨做受的叫床声,国产999热这里只有精品

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>G HA-VSMC人血管平滑肌細(xì)胞

人血管平滑肌細(xì)胞

簡(jiǎn)要描述:CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):G HA-VSMC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-26
  • 訪  問(wèn)  量:2394

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞

ATCC® Number: CRL-1999™ Price: $264.00

Designations: T/G HA-VSMC

Depositors: RR Gifford

Bioped: frozen

Medium & Serum: See Propagation


Growth Propersafety Level: 1

Shipties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast


CRL-1999 T/G HA-VSMC 人血管平滑肌細(xì)胞

Source: Organ: aorta

Tissue: smooth muscle

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applons: tranicatisfection host (technology from amaxa)

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 9,10

THO1: 7,8

TPOX: 8

vWA: 16

Cytogenetic Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.

Age: 11 months

Gender: female

Ethnicity: Caucasian

HeLa Markers: N

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



CRL-1999 T/G HA-VSMC





















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
北辰区| 霸州市| 江川县| 肇州县| 布尔津县| 宜川县| 浑源县| 钦州市| 乌恰县| 灵川县| 比如县| 彩票| 尚志市| 连云港市| 罗田县| 安顺市| 余庆县| 潼关县| 甘肃省| 库车县| 汪清县| 昭苏县| 马龙县| 梅州市| 肇源县| 江孜县| 三门峡市| 淮滨县| 沧州市| 康马县| 宿州市| 屯昌县| 土默特左旗| 凤冈县| 红河县| 万荣县| 宁陵县| 新泰市| 苍溪县| 平昌县| 上饶县|